Njengethuluzi eliyinhloko lokulungiselela isixazululo emalabhorethri, ukuhlanzeka kwamaflaski evolumu kuthinta ngqo imiphumela yokuhlola. Ukugeza ngesandla kwendabuko kubhekene nezinselele ezifana nokusebenza kahle okuphansi, ubunzima bokumisa, kanye nengozi ephezulu yensalela.Imishini yokuwasha amabhodlela ezenzakalelayo ngokuphelele, besebenzisa ubuchwepheshe obufana nokufutha okushisa okuphezulu, ukulawulwa okuhleliwe, kanye nokulandelelwa kwedatha, sekuyinto ebalulekile yamalabhorethri esimanje ukuthuthukisa ikhwalithi yokuhlanza kanye nokusebenza kahle.

I. Izinselele Zokuhlanza Ama-Volumetric Flasks kanye Nezinzuzo Zokwenza Okuzenzakalelayo

1. Imikhawulo Yokuwasha Ngesandla

·Ingozi Yensalela: Isakhiwo sentamo encane samaflaski amakhulu senza kube lula ukuthi aqongeleleke ezinsaleleni zamakhemikhali, okunzima ukuzisusa ngokuzihlanza ngesandla.

·Ukusebenza Kahle Okuphansi: Ukugeza amaflaski ayi-100 kuthatha amahora angaphezu kwama-3, kanye nezikhathi zokomisa ezinde.

·Ukuntuleka Kokujwayelekile: Ukwehluka kwezindlela zokusebenza kwenza kube nzima ukuhlangabezana nezidingo zokuhlanza ezijwayelekile.

2. Izinzuzo Eziyinhloko zeAma-Washer Amabhodlela Azenzakalelayo

·Ukucutshungulwa KweqembuIsibonelo, i-I-XPZI-Aurora F2ithini lokuhlanzaAmaflaski angu-144 x 100mLngomjikelezo owodwa, uqeda ukugeza nokomisa ngaphansiIhora eli-1.

·Ukususwa Kwezinsalela Okusebenzayo: Ihlanganisa40-95°C izinga lokushisa eliphezulu isifuthongezinsipho ezikhethekile zokuhlanza ngokuphelele.

·Ukulandelelwa Kwedatha: Imisebenzi yokuqapha ukuhanjiswa komoya kanye nokuphrinta okukhethwayo iqinisekisa ukuhambisana.

II. Umhlahlandlela Wesinyathelo Ngesinyathelo Wokuhlanza Ama-Flask Anevolumu Ngeumshini wokuwasha ozenzakalelayo

1. Ukwelashwa Kwangaphambi Kokwelashwa Nokulayisha

·Ukuhlunga NokubekaSebenzisa ama-rack ahambisana nosayizi we-flask (5mL–5L), ukuzibekakubheke phansiukuze kufakwe kahle isifutho.

·Ukwelashwa Kwezinsalela Ezinenkani: Kuzinto ezingcolisa izinto eziphilayo (isib. amaprotheni), kunconywa insipho ye-enzymatic.

2. Ukukhethwa Kohlelo kanye Nezilungiselelo Zepharamitha

·Izinhlelo Ezilayishwe Kusengaphambili: I-XPZokunikezwayoIzinhlelo ezijwayelekile ezingama-35 + izinhlelo eziyi-100 ezenziwe ngezifiso, ehlanganisa izidingo eziningi ze-labware.

·Ukulungiswa Ngokwezifiso: Setha ngesandlaizinga lokushisa lamanzi (40–95°C), hlanza ivolumu, bese uphinda umjikelezo.

3. Inqubo Yokuwasha Nokomisa

Isifutho Esinezigaba Eziningi:

Ukuhlanza Ngaphambi KokugezaAmanzi abandayo asusa izinhlayiya ezingaphezulu.

Ukugeza Okuyinhloko:Insipho ye-alkaline + ukujikeleza kwamanzi okushisa okuphezulu ukuze kuqhekeke izinsalela.

Ukuhlanza Kokugcina: Ukungaguquguquki kwamanzi ahlanzekile + i-asidi ukuvimbela ukwakheka kwezikali.

Ukomisa:Ukomisa emoyeni oshisayo (70–105°C)ngokuhlunga okunezingqimba eziningi kuqinisekisa ukuthi akukho ukugcinwa komswakama.