I-Anti-cross-contamination, ukuhlolwa kwe-DNA okuthembekile kungaveza iqiniso emakhoneni afihliwe

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Kuma-movie amaningi kanye nemisebenzi yemibhalo, amalabhorethri e-forensic abonakala njengokuphila okukhethekile nokubalulekile, ikakhulukazi isiqephu sokuhlola ukuhlonza i-DNA ngokuvamile siba ukhiye wokuthola imikhondo nokuxazulula amacala.Nokho, uma ukunemba kwemiphumela yokuhlolwa kwethulwa kungatshazwa, ngokwemvelo ngeke kube ubufakazi obungokomthetho, ingasaphathwa eyokuveza iqiniso emakhoneni afihliwe.Kunesici esikhethekile somzimba nesamakhemikhali okufanele izilabhorethri zesayensi yezomthetho ziyiphathe ngokuqapha, futhi lokho ukuvimbela amasampula e-DNA azohlolwa ukuthi angangcoli elabhorethri.Njengamanje, kunezimbangela ezahlukene zokungcoliswa kwe-DNA.Phakathi kwabo, kungenzeka ukuthi ukutheleleka ngegciwane kukhulu kakhulu.
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Njengamanye amalabhorethri, izinto ezisetshenziswayo kanye nemishini kumalabhorethri e-forensic kungcolile ezimweni eziningi.Ikakhulukazi, ukuthelelana ngezifo phakathi kwamasampuli e-DNA okusekelwe ekuphenduleni kwe-PCR, ezinye izinto zokuhlola nobufakazi obuphathekayo, kanye nabahloli ngokwabo yibo okunzima kakhulu ukukubona.Lezi zinsalela zokungcola zihlanganisa amangqamuzana e-biological, igazi, izicubu, kanye nama-reagents okuhlola, izinto zokugeza izitsha nokunye ukungcola.

Kuyafaneleka ukugcizelela ukuthi i-glassware engasetshenziswa kabusha kumalabhorethri e-forensic njengeziqukathi zesampula, amabhodlela e-reagent, amashubhu okuhlola, ama-pipette, ama-flasks, izitsha ze-petri, njll. Ukuqaliswa kwazo okunganele, ukungahambisani, nokungathobeli emisebenzini yokuwasha kungenye yezinto izigebengu eziholela ekuhlonzweni okungalungile kanye neziphetho zokuhlaziya.

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Lolu hlobo lokungcoliswa kwe-glassware luwusongo olusobala emiphumeleni yokuhlolwa, ngakho-ke yini ukhiye wokukuxazulula?

Okokuqala, lapho kutholakala isimo esisolisayo sokungcoliswa kwe-DNA, imiphumela yokuhlolwa kufanele iphinde ihlolwe kusenesikhathi ukuze kuphinde kutholakale iphutha.Lena into ehamba phambili yokuqala.

Bese, yenza ukulandelelwa kokulawula ikhwalithi ezintweni ezisetshenziswayo zokuhlola, okuhlanganisa iziqukathi zengilazi, ama-reagents, njll., ukuze uqinisekise ngokuqhubekayo umthombo wokungcola.

Ngalesi sisekelo, thuthukisa izinqubo zokuhlanza ze-glassware ukulungisa amaphutha, ukuze ugweme amaphutha afanayo ukuthi angenzeki futhi.

Okwesithathu, kuphela ngokuqinisa zonke izinyathelo zokulwa nokungcoliswa kanye nokuqeda ukungcola kwelabhorethri ukuze kwakheke abaphathi besikhungo lapho ukuthuthukiswa kwenqubo yokuhlanza kungaba nenjongo futhi kuphumelele.

Eqinisweni, ilabhorethri yezobunhloli efanelekayo kufanele ibe nendawo ezinikezele futhi ezimele yokuhlolwa kwe-DNA ukuze kuqinisekiswe ukuthi ukungcola kuyancishiswa ezixhumanisini ezihlukene zokuhlola.Isibonelo, indawo yokwamukela icala kanye nendawo yokugcina isampula, indawo yokukhipha isampula ye-DNA, indawo yokukhulisa i-DNA, indawo yokuthola i-DNA, indawo yokuhlolwa kokuqala, indawo yokuhlaziya umphumela, indawo yokulungiselela, indawo yokukhulisa i-DNA, indawo ye-buffer yokuthola, njalo njalo.Phakathi kwabo, ukuhlanza i-glassware endaweni yokulungiselela kuzosiza kakhulu ukwandisa amathuba okuhluleka kwemiphumela yokuhlolwa.

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Kumele kuqashelwe ukuthi ama-laboratories amaningi ochwepheshe, kuhlanganise nama-laboratories e-forensic, asasebenzisa izindlela zokuhlanza ezingasebenzi kahle zokuxazulula inkinga yezinsalela zokungcola ku-glassware.Kodwa le ndlela ayithuthukisi ubungozi bokuthi abantu bangenwe yizifo ezahlukene.

Ngaphezu kwalokho, ububi bokuhlanza ngesandla kwe-glassware budlula lokhu.

Mukuhlanzwa kwe-glassware nge-anual ngeke nje kwehluleke ukuhlanza ngokuphelele i-glassware yaselabhorethri futhi kuthinte isiphetho sokugcina sokuhlolwa kwe-DNA nokuhlonza, kuzophinde kulethe uchungechunge lwezinto eziphikisanayo ezifana nokumosha izinsiza, inkimbinkimbi yokuhlanza imisebenzi, kanye nezingozi zokuphepha kubasebenzi baselabhorethri. .Ngalesi sikhathi, ukusetshenziswa kwe- othomathikhi glassware washeresetshenziswa kumalabhorethri e-forensic yomhlaba wonke izosiza ukwenza ngcono lesi simo.

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Iwasha ingilazi yelabhorethriingakwazi ukuhlanza ngokunembile izinhlobonhlobo zezingilazi zaselabhorethri ngendlela ephephile, inqwaba, futhi ehlakaniphile ukuze iyenze ihambisane nemithetho efanele ye-GMP kanye ne-FDA.Uma kuqhathaniswa nendlela yokuhlanza ngesandla, i Iwasha Labhorethriingakwazi ukulandelela inqubo yokuhlanza kuyo yonke inqubo, esiza ekutholeni amarekhodi edatha abalulekile ezicini ezihlukahlukene.Le datha ibaluleke kakhulu ekususweni kwezimo eziyinsalela okuhlanganisa nokungcoliswa okubhekene nokuhlolwa kwe-DNA.Ikakhulukazi uma kukhona umehluko nokungabaza mayelana nemiphumela yokuhlolwa!

Ngokuthuthuka okuqhubekayo kobuchwepheshe obufanele, amalabhorethri e-forensic azothatha imithwalo yemfanelo eyengeziwe enqubweni yokusingatha amacala.Ngale ndlela, izidingo zanoma yimiphi imiphumela yokuhlolwa kwaselabhorethri kanye nokumiswa kanye nokunemba ngokwemvelo zizoqhubeka nokwanda.Izindlela ezihlanganisa ukuhlola i-DNA zingaqinisekisa kuphela imiphumela ehlanzekile futhi zithole iziphetho ezifanele kanye nobufakazi uma ziphumelela ekukhipheni ukungcola.Lokhu kuyinto okufanele yonke ilabhorethri ye-forensic ikhumbule.


Isikhathi sokuthumela: Apr-02-2021